--- title: "Kinase phosphoryl transfer (hexokinase) Template | MolDraw" url: https://www.moldraw.com/templates/enzyme-kinase-phosphoryl-transfer/ description: "Kinase phosphoryl transfer (hexokinase) template: Hexokinase: an Asp side chain removes the proton of the glucose C6–OH as it attacks the γ-phosphorus…" lang: en publisher: Scidart Academy generated: 2026-11-13 --- [MolDraw](https://www.moldraw.com/) › [Templates](https://www.moldraw.com/templates/) › [Biochemistry](https://www.moldraw.com/templates/biochemistry/) › Kinase phosphoryl transfer (hexokinase) [Biochemistry](https://www.moldraw.com/templates/biochemistry/)Premium # Kinase phosphoryl transfer (hexokinase) template ![Kinase phosphoryl transfer (hexokinase) template: Hexokinase: an Asp side chain removes the proton of the glucose C6–OH as it attacks the γ-phosphorus opposite the β,γ-bridging oxygen (in-line, inversion at P), and ADP leaves; Mg²⁺ bound to the β- and γ-phosphates shields their charge. In ATP hydrolysis water is the nucleophile.](https://www.moldraw.com/templates/enzyme-kinase-phosphoryl-transfer.webp) [Edit in MolDraw](https://www.moldraw.com/)Download with Pro Premium template. Preview and edit it free in MolDraw; downloading and exporting it is part of [MolDraw Pro](https://www.moldraw.com/pricing). Hexokinase: an Asp side chain removes the proton of the glucose C6–OH as it attacks the γ-phosphorus opposite the β,γ-bridging oxygen (in-line, inversion at P), and ADP leaves; Mg²⁺ bound to the β- and γ-phosphates shields their charge. In ATP hydrolysis water is the nucleophile. ## Mechanism step by step In-line attack on the γ-phosphorus of MgATP²⁻: glucose 6-OH becomes glucose 6-phosphate; water does the same in ATP hydrolysis 1. **Phosphoryl transfer** – Asp (base) takes the 6-OH proton; O6 attacks Pγ in line with the leaving β,γ-bridge oxygen 2. **ATP hydrolysis** – The same in-line attack with water as the nucleophile (ATPases, myosin) ## Key points - The true substrate is MgATP²⁻: Mg²⁺ on the β- and γ-phosphates shields the negative charge so the nucleophile can approach Pγ. - In-line attack goes through a trigonal-bipyramidal transition state and inverts the configuration at P (shown with chiral [¹⁶O,¹⁷O,¹⁸O]-phosphoryl groups). - Hexokinase closes around glucose (induced fit, Koshland), which shuts water out of the site so ATP is not simply hydrolysed. - ATP hydrolysis: ΔG°′ ≈ −30.5 kJ/mol; in cells ΔG is about −50 to −65 kJ/mol because [ATP]/[ADP][Pᵢ] is held far from equilibrium. Drawn with 153 atoms, 9 arrows, 6 shapes – every one editable in MolDraw. ## Sources - [Nelson & Cox, Lehninger Principles of Biochemistry, 8th ed., 2021, ch. 14](https://openlibrary.org/isbn/9781319228002) Textbook - [Berg, Tymoczko, Gatto & Stryer, Biochemistry, 9th ed., 2019, ch. 9](https://openlibrary.org/isbn/9781319114657) Textbook ## How to use this template 1. Press **Edit in MolDraw**. The template opens on the canvas of the free MolDraw editor. 2. Change anything: move structures, edit atoms and labels, recolour highlights or delete what you do not need. 3. Add your own structures, arrows and text next to it, or combine several templates on one page. 4. Export the drawing as PNG, SVG or PDF for slides, worksheets, theses and papers. ## Frequently asked questions ### Is the Kinase phosphoryl transfer (hexokinase) template free? You can preview the Kinase phosphoryl transfer (hexokinase) template here and open and edit it in MolDraw for free. Downloading or exporting it is part of MolDraw Pro, together with the other premium templates. ### Can I edit the Kinase phosphoryl transfer (hexokinase) template? Yes. It opens as an ordinary MolDraw drawing, so every structure, arrow, label and colour can be changed, and you can add your own content around it. ### Is the chemistry checked? Every structure is checked with RDKit and every curly arrow passes MolDraw’s electron-flow audit. The sources used are listed on this page. ### Are the biochemical pathways drawn with structures? Yes. 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